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Food Microbiology

Food Microbiology: obtain faster and more reliable results in the most economical way possible. Also for food facility microbiology.

Food Microbiology: At MICROKIT we have been designing food microbiology products for 34 years to help factories obtain faster and more reliable results in the most economical way possible. We are also specialists in food facility microbiology, with kits that factories can use even if they do not have a self-control laboratory.

https://www.microkit.es/eng/fichas/EXCLUSIVE%20MICROKIT%20PRODUCTS%20FOR%20FOOD%20ANALYSIS,%20WITH%20THEIR%20DIFFERENTIAL%20ADVANTAGES.pdf

 

A) Food control products

Detect Salmonella and Listeria in food reliably, in just 36 hours and without the need for special equipment:

https://www.microkit.es/eng/fichas/Listeriquick%20&%20Salmoquick%202021%20EN.pdf

All the classic culture media you need for food microbiology:

https://www.microkit.es/eng/fichas/MICROKIT%20media%20EN.pdf

All the chromogenic culture media you need for food microbiology:

https://www.microkit.es/eng/fichas/cromokit%20chromogenic%20media%202021%20EN.pdf

The best isolement agar to detect Salmonella in food:

https://www.microkit.es/eng/fichas/CROMOSALM%20EN.pdf

…after using the best enrichment broth:

https://www.microkit.es/eng/fichas/SALMONELLA-SHIGELLA%20MICROKIT%20BROTH.pdf

The best medium to detect Listeria in food:

https://www.microkit.es/eng/fichas/Cromocytogenes%20EN.pdf

The best medium of detecting and enumerating E. coli and other coliforms in foods:

https://www.microkit.es/eng/fichas/mugplus%20EN.pdf

Tired of food artifacts in PCA and not being able to distinguish colonies from the medium?

https://www.microkit.es/eng/fichas/pca%20cromogenic%20EN.pdf

Enumerate yeasts and molds in just 18-36 h with Rapid Sabouraud RYM Agar

https://www.microkit.es/eng/fichas/RAPID%20SABOURAUD%20RYM%20AGAR.pdf

Eliminate the matrix effect (and its false negatives) due to natural (flavoring) and artificial food preservatives, thanks to the MICROKIT patented Buffered Peptone Neutralizing Water

https://www.microkit.es/eng/fichas/BUFFERED%20PEPTONE%20NEUTRALIZING%20WATER%20EN.pdf

Confirm suspicious colonies without spending expensive identification galleries:

https://www.microkit.es/eng/fichas/m%20ident%20EN.pdf

The most convenient method of analysis for the food industry, dehydrated plates for both strike and pour plate methods:

https://www.microkit.es/eng/fichas/Dry%20Plates%202021%20EN.pdf

B) Food facility control

Purify the air and surfaces of your factory (in the absence of people and food for 3 hours)

https://www.microkit.es/eng/fichas/Airesano%20EN.pdf

Validate the hygiene of your surfaces with the immediate response kit that does not require any equipment:

https://www.microkit.es/eng/fichas/kitpro%20PLUS%20EN.pdf

Use MICROKIT’s best-selling kit to comply with EU regulations on Listeria on surfaces, without the Aesculin false positives of other kits:

https://www.microkit.es/eng/fichas/listeriswabs%20green%202021%20EN.pdf

Control the count of Aerobes, Fungi, Enterobacteria, Coliforms, Salmonella… on surfaces with the most classic MICROKIT kit (dipslides):

https://www.microkit.es/eng/fichas/desinfectest%20EN.pdf 

Verify that the waters that reach your factory have not suffered infiltration by wastewater or natural water, with MICROKIT P/A kits:

https://www.microkit.es/eng/fichas/P-A%20powder%20vials%202021.pdf

Are you not happy with any of the microbiological parameters you analyse? Contact us, we are sure you will be surprised by our answer. That is why we have been designing analysis media and kits for 34 years and coordinating intercomparative food microbiology services for 25 years.

 

https://www.microkit.es/eng/fichas/MICROKIT%20export%202024%20Catalogue.pdf

Our innovation is yours. And so is our know-how: export@microkit.es

If you want to order some MICROKIT product, please send your order to: pedidos@microkit.es

Water Microbiology

Water Microbiology

Water Microbiology: Consuming water, bottled water, bath water, natural waters… require large sample volumes (generally 100 or 250 mL) in the search for pathogens and their indicators.

The most widely used method for working with such samples is Membrane Filtration (MF). In this method, these volumes are filtered and the filter is applied to the culture medium plate, so that colonies grow if the microorganisms sought are present.

https://www.microkit.es/eng/fichas/plaquis%20hermetic%20EN.pdf

Another very universal method is the Most Probable Number (MPN), in which several tubes or wells of liquid culture medium are used to extrapolate the count of the microorganisms sought, according to the number of positive tubes or wells (which change color).

And the third most widely used method is the Presence/Absence (P/A) method in which the microorganisms are not enumerated and the search is made to see if they are present or not in the sample by changing or not the color of the water. Since in all cases, we are looking for 0 to declare the water as suitable or >1 to declare it as unsuitable, obtaining a count can therefore be considered redundant.

The only parameter for which only 1 ml of water sample is often analyzed is the aerobic count.

MICROKIT offers everything you need in water analysis, whatever method you use, and for all microbiological parameters. And using, as we use, the three methods, which is our favorite? We will see it throughout the text. Let’s look at the methods and their solutions one by one:

 

Total count in 1 mL of water sample (Water Microbiology)

Normally, 1 mL of water sample is added to a Petri dish, and after melting and cooling the solid medium to 45-47ºC, 15-25 mL of agar are added (sowing by mass inclusion).

The most commonly used media are YEA-Nutrient Agar (PCA-water) in water for human consumption (ISO 6222) and R2A in Pharmacopeial water.

Many laboratories use food microbiology variants such as PCA (although it is not suitable for the water oligotrophs such as YEA and R2A) or the general medium they have on hand, such as TSA.

Many laboratories use our chromogenic YEA (because the colonies grow much more clearly red in contrast to the cream medium). They even use it in the most convenient version that exists (DryPlates-TC Water), because it does not require melting and cooling media for the 1 mL of water to be sown in mass. And so the analysis time is reduced from 1 h to 10 seconds.

https://www.microkit.es/eng/fichas/Dry%20Plates%202021%20EN.pdf

In cooling waters, whether natural or contaminated ones, dipslides such as our Desinfectest-TC can be used, since they provide an estimate of the aerobic load (10E3, 10E4, 10E5…) without having to make dilutions.

https://www.microkit.es/eng/fichas/desinfectest%20EN.pdf

The rest of parameters usually requires samples of 100-250 mL:

 

MF Method for Water Microbiology

For Coliforms/E. coli, the CCA chromogenic medium is used, which we at MICROKIT christened MugPlus in 1995. This is a great advance over the previous official medium (Tergitol TTC), especially in acidic waters. The blue colonies are of E. coli and the pink ones of the other coliforms. The blue ones can be confirmed immediately with Kovacs indole (+) on the same plate in the MICROKIT medium and the pink ones with oxidase reagent (-) also on the same plate.

https://www.microkit.es/eng/fichas/mugplus%20EN.pdf

For faecal Enterococci, Slanetz-Bartley Agar (SB, red colonies) is used followed by Bile Esculin Azide Agar (BEA, white colonies with a black halo). At MICROKIT we have validated our Rapid-BEA so that the filter can be used directly on this medium, avoiding the previous step through SB, which allows us to give results in 18 hours instead of the usual 48 hours. And for those who prefer chromogenic media, our EPA medium has the same performance, with dark blue colonies.

For Clostridium perfringens and its spores, TSC Agar (black colonies) and Acid Phosphatase are used to confirm them (preferably in its fluorescent MUP version, because it is not carcinogenic). At MICROKIT we also offer the chromogenic medium (orange colonies unlike TSC, which do not revert after removing the plate from the anaerobic atmosphere). Despite being a parameter of maximum importance (the two previous ones indicate contamination by waste water, but this one indicates contamination by natural waters, often with protozoa such as Cryptosporidium), the intercomparative services show that the poor results (50% false negatives and a 1-3 log drop in the counts) are not due to the medium, but to the MF method, because it oxygenates the anaerobes in the water in a way that is lethal to them. At MIcrokit we have invented a magnificent solution with the same TSC medium that we will see later (Quanti-P/A).

For Pseudomonas aeruginosa (bottled water, bathing water, water for hospital consumption…), Cetrimide Agar CN is used (lobed colonies, often phosphorescent, yellow, green or blue). At MICROKIT we also have the chromogenic medium that gives results in just 18 hours instead of the 48 hours of the classic CN: alert in 18h by red colonies, confirmed with fluorescent halo in 48h. If no red colonies in 18h, no Pseudomonas aeruginosa are present.

For bacteriophages (somatic coliphages) must be used Scholten Agar and search for and look for growth plaques of the E. coli strain inoculated on the plate. It is a new parameter of the latest renewal of EU legislation 1/2023 that is an excellent indicator of traveller’s diarrhoea, micro-epidemics… At MICROKIT we also offer the other 5 media required by ISO 10705 for these and the other bacteriophages mentioned therein.

https://www.microkit.es/eng/fichas/BACTERIOFAGOS%20SCHOLTEN%20AGAR.pdf

Water Microbiology

 

MPN Method

For Coliforms/E. coli, there is an ISO that talks about a medium with a defined substrate, but our Colicult is more recent and better because it also detects enterotoxigenic E. coli. Coliforms turn 100 mL of water blue and E. coli fluoresces blue in the dark. It can also be confirmed directly with Indol Kovacs in seconds. You can use the cuvettes from this ISO or also our own (NMP-Racks) with our own NMP tables.

For fecal Enterococci there is also an ISO with a fluorogenic medium, but our Enterocult broth (based on BEA) is newer and better. ISO cuvettes or our NMP-Racks can also be used.

For Clostridium perfringens and its spores, this NMP Method does not work well, because it requires an anaerobic atmosphere. That is why at MICROKIT we invented the Quanti-P/A bags with TSC, cold gelling agent and an anaerobic atmosphere. 100 mL of sample water are introduced, mixed with the medium by kneading, incubated, and the black colonies are observed and enumerated.

https://www.microkit.es/eng/fichas/QUANTI-PA%20Clostricult%20EN.pdf

For Pseudomonas aeruginosa you can use the USA fluorescence method with the aforementioned cuvettes. Or better yet, our Pseudocult, which in addition to fluorescence, turns pink in positive wells. Our cuvettes NMP-Racks can also be used.

Water Microbiology

 

P/A Method

Although for us it is the most logical method (why to be counting zeros?), easier to use and more reliable, some legislations have relegated it to work in laboratories not subject to ISO standards and to field work (for natural waters, wells, taps…), which is no small feat.

The same broths mentioned in the NMP method, are used to determine whether or not coliforms-E. coli, fecal Enterococci, Pseudomonas aeruginosa and many other parameters are present. No cuvettes are necessary. Simply add the 100 mL of water sample to the medium, incubate it and observe whether there is a color change (+) or not (-).

For Clostridium perfringens and its spores, Clostricult P/A is sufficient without the need to use Quanti-P/A TSC; this method also does not require anaerobic incubation. Black (sometimes only the bottom of the container): positive, otherwise: negative.

This method is so successful at MICROKIT that we have designed 3 formats, to choose from depending on the convenience, number of samples or budget of each client:
1-The classic sample bottle, with the sterile medium already hydrated, to add the 100 mL of water directly into it

https://www.microkit.es/eng/fichas/P-A%20kits%20in%20sampler%20bottles%20EN.pdf
2-The economical format for larger consumers: pre-weighed vials of sterile powder medium

https://www.microkit.es/eng/fichas/P-A%20powder%20vials%202021.pdf
3-The very economical format in 100 g jars of sterile powder medium: add a teaspoon to 100 mL of water sample and close the rest of the jar for later uses

https://www.microkit.es/eng/fichas/P-A%20Kits%20in%20100%20g%20bottles%20EN.pdf

Water Microbiology

We also offer kits that contain the P/A and DryPlates components necessary for the analysis of each type of water:

A) Water for human consumption

https://www.microkit.es/eng/fichas/drinking%20water%20kit%20EN.pdf

B) Bath water

https://www.microkit.es/eng/fichas/swimming%20pool%20kit.pdf

C) Water for cosmetic use

https://www.microkit.es/eng/fichas/COSMETIKIT%20WATER%20EN.pdf

 

Others

And we also have quantitative strains, confirmation kits, bottles and bags, membranes, and everything necessary for any laboratory, regardless of size, to be able to analyse its water samples in the way that best suits it.

https://www.microkit.es/eng/fichas/QUANTITATIVE%20MICROBIOLOGICAL%20STRAINS%202017.pdf

https://www.microkit.es/eng/fichas/m%20ident%20EN.pdf

https://www.microkit.es/eng/fichas/Exclusive%20MICROKIT%20products%20for%20water%20with%20their%20differential%20advantages.pdf

https://www.microkit.es/eng/fichas/MICROKIT%20export%202024%20Catalogue.pdf

 

Request more information and prices at export@microkit.es

If you want to order some MICROKIT product, please send your order to: pedidos@microkit.es

COSMETICS MICROBIOLOGY

COSMETICS MICROBIOLOGY

Evolution of cosmetic microbiology: From Pharmacopoeia to ISO Standards for cosmetic microbiology. Reasons why cosmetics are still being recalled. Even by large companies. Solutions to avoid such recalls and sanctions.

Not many years ago, cosmetic microbiology was the same as pharmaceutical microbiology. But the first thing the Pharmacopoeia says is that it is validated for medicines (i.e. not for cosmetics).

Once this reality was confirmed, MICROKIT designed the first proficiency service for cosmetics microbiology in 2005.

And indeed, the results were devastating: almost all participating laboratories obtained yields of only 40 to 60%.

That is, out of every 10 analyses, between 4 and 6 false positives or false negatives were obtained.

It was very clear that Pharmacopeia was not useful for cosmetics.

Shortly afterwards, the ISO Standards for cosmetics microbiology were approved and 3 mistakes were made in them:

1) Too closely emulating the Pharmacopeia method, which gave such poor results in cosmetics

2) Focusing on 4 pathogens/indicators (Candida albicans, Staphylococcus aureus, E.coli and Pseudomonas aeruginosa) and forgetting about emerging pathogens (since ISO 18415 on «specified and unspecified microorganisms» does not solve the problem; because simply strikes enriment broth in TSA, In which harmless microorganisms also grow. This waste the time of laboratories, which are forced to identify them all even if they are not pathogenic.).

3) And they also forgot about the critical waters for cosmetic use, in which no one at the water treatment plant looks for the microorganisms that cause the most problems in cosmetics. Because the legislation on water for human consumption does not contemplate them

The results of the laboratories in successive rounds of these cosmetic intercomparative services (once the ISO Standards for cosmetic microbiology were implemented in many of its participants), were better than those of the Pharmacopoeia, but still had much room for improvement. Especially in highly ubiquitous emerging pathogens, like Burkholderia cepacia or Pluralibacter gergoviae.

Time has shown that there were up to 10 emerging pathogens to add to the 4 with specific ISO standards. Pathogens that created numerous and high-profile market recalls. Because only health authorities were looking for them, not the cosmetics industries or the external laboratories to which they entrusted their analyses.

It was understandable that the cosmetics industry is opposed to adding 10 new culture media to its analyses in addition to the 4 they already used, in order to guarantee that its cosmetics are microbiologically safe and harmless, as required by EC Regulation 1223/2009 (the only legislation on the subject).

But what if you only needed to add one culture medium to detect the remaining 10 pathogens? Without false positives of harmless ones (like TSA) or false negatives.

This idea had been floating around in MICROKIT’s head for a long time. And after overcoming the fears of what seemed impossible at first sight, MICROKIT achieved it!

A single culture medium that detects the 10 emerging pathogens that do not have an associated ISO standard (and also two of those that do).

Could you ask for more?

This magic and blessed medium has been called CUP12A (Cosmetic Universal 12 Pathogen Agar) and it’s available since 2023.

https://www.microkit.es/eng/fichas/CROMOKIT%20CUP-12%20AGAR%20Cosmetic%20Universal%20PathoChrom%20Agar.pdf

You just have to add it to the 4 that everyone uses, to be sure that you will not continue to suffer the uncertainty of a probable market withdrawal.

On the other hand, we offer the neutralizing broth that obtains the best results when used in intercomparison. Another fruit of these 15 years of intercomparing results between cosmetic laboratories: LPTN Broth (derived from Eugon and Modified Letheen, but with doping factors and with neutralizers of all current authorized preservatives).

https://www.microkit.es/eng/fichas/LPT-NEUTRALIZING%20BROTH%20COLOURLESS.pdf

And to speed up aerobic and fungal counts (in just 18-36h) we offer two other magical media: RYM Agar and Maxim Agar (a chromogenic PCA derivative):

https://www.microkit.es/eng/fichas/RAPID%20SABOURAUD%20RYM%20AGAR.pdf

https://www.microkit.es/eng/fichas/pca%20cromogenic%20EN.pdf

Finally, for manufacturers who do not yet have their own self-control laboratory, we have designed a prepared media kit containing everything we have described above (including all the necessary consumables), the COSMETIKIT-EASYPLUS. You only need a culture incubator and this kit to be able to start performing your own analyses of the 14 pathogens, since the kit also includes the neutralization of preservatives and the counting of aerobes and molds:

https://www.microkit.es/eng/fichas/COSMETIKIT%20EASYPLUS%20EN.pdf

 

And for those who want to follow more thoroughly the GMPs reflected in ISO 22716 (mandatory implementation in cosmetic factories), we offer the complete microbiological water analysis kit, which includes the most important parameters of water potability, and also the two emerging ones that cause the most problems in cosmetic waters: Pseudomonas aeruginosa and Burkholderia cepacia: COSMETIKIT WATER:

https://www.microkit.es/eng/fichas/COSMETIKIT%20WATER%20EN.pdf

 

We also offer all the quantitative strains needed for quality control and high-load strains for Challenge Test. Request a stock list with exact loads and expiration dates:

https://www.microkit.es/eng/fichas/QUANTITATIVE%20MICROBIOLOGICAL%20STRAINS%202017.pdf

 

And to validate the cleanliness of your facilities, use the simplest kit, with very evident results, which does not require any equipment; and with an immediate response, by turning green, when organic matter poses a risk of microorganism accumulation, the KitProPlus:

https://www.microkit.es/eng/fichas/kitpro%20PLUS%20EN.pdf

Another advancement we offer is the hermetic prepared plates with a long shelf life (5 real months) and the dehydrated plates with a shelf life of 1-2 years:

https://www.microkit.es/eng/fichas/plaquis%20hermetic%20EN.pdf

https://www.microkit.es/eng/fichas/Dry%20Plates%202021%20EN.pdf

https://www.microkit.es/eng/fichas/MICROKIT%20export%202024%20Catalogue.pdf

 

Take advantage of all of MICROKIT’s experience in cosmetic microbiology (34 years of innovation). MICROKIT, your best ally; ask us what you need to know at: export@microkit.es

If you want to order some MICROKIT product, please send your order to: pedidos@microkit.es

 

MICROBIOLOGICAL STRAINS

OPTIMIZED CONSERVATION OF MICROBIOLOGICAL STRAINS

MICROBIOLOGICAL STRAINS: 1-OPTIMIZED CONSERVATION OF IN HOUSE STRAINS AND ALSO  STRAINS GROWN IN YOUR ANALYSIS. 2-USE OF QUANTITATIVE STRAINS

 

1-OPTIMIZED CONSERVATION OF IN HOUSE STRAINS AND ALSO  STRAINS GROWN IN YOUR ANALYSIS.

Only a few decades ago, strain preservation was done by reseeding them weekly or monthly in long-nose slant CTA medium tubes.

In many laboratories, with large strain libraries for epidemiological studies or for R&D, this required the full-time work of a research analyst.

Pharmacopoeia later urged the use of TSB with glycerol in cryovials for freezing, which allowed resowing to be prolonged for 1 year in many strains (although not in others).

By adding porous porcelain beads, similar to those used by children in coloured bead necklaces, a re-seeding was avoided every time the strain had to be used.

Since the cryovial could be re-frozen with the rest of the beads, once the one needed to re-seed the strain had been extracted. This is how the word «cryoballs» was born.

But there were still many microorganisms that did not hold up well in these conditions. And became unviable after being put in and taken out of the freezer several times, or simply after a few months.

This is when MICROKIT came along in 1989 and, with the help of its best large strain library customers, created several solutions that have proven to be very successful:

1-Crioteca-Skim Milk. Its composition allows for a much longer lifespan for most of the microorganisms stored in strain libraries than other criovials made with TSB-glycerol

https://www.microkit.es/eng/fichas/crioteca%20english.pdf

2. Vibrio cholerae continued to resist and after many tests, we created MPT Agar tubes that allow its reseeding to be delayed only once a year, with conservation at room temperature

3-Campylobacter spp. also resisted and thanks to CRIOTECA-Campy we achieved much better results

4-Fungi (yeasts and moulds) also remain viable for much longer if they are frozen in the CRIOTECA-YM

5-And anaerobes such as Clostridium, resist excellently at the bottom of ANAEROTECA tubes for years, at room temperature

The cryovials we use in our CRIOTECA are the best we have found, with a truly hermetic seal, a cap large enough to be opened/closed properly. With the thread on the outside of the vial so as not to contaminate, etc.

In addition, their size allows 100 units to be stored in the space where 40 units of other brands fit; and we already know which is the most expensive space in a laboratory: the freezer. In addition, we serve them in top quality boxes (CRIOBOX) made of transparent plastic.

The CRIOTECA, ANAEROTECA and MPT kit set is probably the line of kits with the most units sold per year by MICROKIT.

And with the most satisfied customers, who come to us without knowing them, thanks to word of mouth from those who already use them.

OPTIMIZED CONSERVATION OF MICROBIOLOGICAL STRAINS

 

2-USE OF QUANTITATIVE STRAINS

On the other hand, we design and manufacture Quantitative Strains (currently the 73 most used by our clients in the food, cosmetic, pharmaceutical and water industries).

https://www.microkit.es/eng/fichas/QUANTITATIVE%20MICROBIOLOGICAL%20STRAINS%202017.pdf

The great advantage of our lentil-shaped quantitative strains is their tremendous longevity: 2 years of manufacturing, twice as long as any other brand.

They are so stable that they do not need frozen transport, not even in cold storage, since they can last for weeks at room temperature without changing.

In addition, their price is very competitive.

Used by numerous ISO 17025 accredited laboratories, our quantitative strains are accompanied by a very complete quality control certificate, which includes:

-reference to the universal collection of ISO 11133-2 (WDCM)

-traceability to the type collection,

-passage number from the type collection,

-count and deviation (CV%) within the batch. Which are done in TSA/SDA. And also in at least one selective medium (the most commonly used in each microorganism). And often in a third chromogenic selective medium.

https://www.microkit.es/eng/fichas/MICROKIT%20export%202024%20Catalogue.pdf

Please ask us for documentation and prices to: export@microkit.es

If you want to order some MICROKIT product, please send your order to: pedidos@microkit.es

Chemical microbiology

Chemical microbiology: problems and solutions

The chemical industry often needs kits to control the microbiological load that can alter its products and facilities. At MICROKIT we have designed many solutions for these industries:

 

1-Dipslides to determine the bacterial and fungal load in liquids that are susceptible to being altered when the microorganism load increases: DESINFECTEST-MIX

https://www.microkit.es/eng/fichas/desinfectest%20EN.pdf

 

2-Tubes to detect sulphate reducers, which corrode the metals in the facilities and generate unbreathable hydrogen sulphide gas: SULFATE API AGAR TUBES

 

3-Tube kits with swabbs to quickly detect where bacteria accumulate (from the first 4 hours): RAPIDSWABS-TC

 

4-Tube kits with swabbs to quickly detect where fungi (yeast & moulds) accumulate (from the first 18 hours): RAPIDSWABBS-RYM

https://www.microkit.es/eng/fichas/RAPID%20SABOURAUD%20RYM%20AGAR.pdf

 

5-For a more accurate count of the different microorganisms in your products, we designed the DryPlates range, dehydrated prepared plates with a very long shelf life:

https://www.microkit.es/eng/fichas/Dry%20Plates%202021%20EN.pdf

 

6-And if you need to analyze the potability of your plant’s water, or even more microbiological parameters, we also have the best solution: DrinkingWaterKIt and P/A Kits. Also the hermetic plates for Membrane filtration

https://www.microkit.es/eng/fichas/drinking%20water%20kit%20EN.pdf

https://www.microkit.es/eng/fichas/P-A%20powder%20vials%202021.pdf

https://www.microkit.es/eng/fichas/plaquis%20hermetic%20EN.pdf

 

7-We can also help you to immediately detect the sources of organic matter that will cause microbial accumulations in the coming days:

https://www.microkit.es/eng/fichas/kitpro%20PLUS%20EN.pdf

 

We have many other specific solutions in Chemical microbiology for different microorganisms: ask us what your problems are, and we will solve them for you. Write us to: export@microkit.es

If you want to order some MICROKIT product, please send your order to: pedidos@microkit.es

https://www.microkit.es/eng/fichas/MICROKIT%20media%20EN.pdf

https://www.microkit.es/eng/fichas/MICROKIT%20export%202024%20Catalogue.pdf

 

Microbiology

Microbiology: Microbiological problems? 10 problems, 10 MICROKIT solutions

There are 10 problems in microbiology that affect many laboratories. MICROKIT has created ingenious solutions for all of them, easy to implement and that optimize analytical results.

 

1-Microbiological strains conservation:

From weekly reseeding in tubes with a spout to conservation for years: Skim Milk Cryoteca, Anaeroteca and MPT-Vibrio cholerae inclined tubes

https://www.microkit.es/eng/fichas/crioteca%20english.pdf

 

2-Disinfection of laboratory air and surfaces:

Fog without the typical UV shadows: Airesano

https://www.microkit.es/eng/fichas/Airesano%20EN.pdf

 

3-Surface control:

Microbiological control of surfaces: Various alternatives to Rodac plates and dipslides for all types of surfaces: Rapidswabs-TC, Rapidswabs-YM, Sulfate API Agar tubes, Listeriswabs-Green…

https://www.microkit.es/eng/fichas/desinfectest%20EN.pdf

https://www.microkit.es/pdf/Listeriswabs-green-2021.pdf

 

4-Surfaces-Hygiene:

The control/Validation of cleaning organic matter on surfaces required devices: KitProPlus regarding bioluminescence, it is like Uber regarding taxis

https://www.microkit.es/eng/fichas/kitpro%20PLUS%20EN.pdf

 

5-Cosmetics Microbiology:

There are not 4 pathogens, it is the absence of 14 that guarantees the microbiological safety of your cosmetics, as required by legislation (EC Regulation No. 1223/2009). Start your self-control with Cosmetikit-EasyPlus, and veteran laboratories, using a single additional medium: CUP12A.

https://www.microkit.es/eng/fichas/CROMOKIT%20CUP-12%20AGAR%20Cosmetic%20Universal%20PathoChrom%20Agar.pdf

https://www.microkit.es/eng/fichas/LPT-NEUTRALIZING%20BROTH%20COLOURLESS.pdf

https://www.microkit.es/eng/fichas/COSMETIKIT%20EASYPLUS%20EN.pdf

https://www.microkit.es/eng/fichas/COSMETIKIT%20WATER%20EN.pdf

https://www.microkit.es/eng/fichas/Dry%20Plates%202021%20EN.pdf

 

6-Food Microbiology:

Listeria and Salmonella detection is slow or expensive with devices: economical detection in just 36 hours and without special devices: Listeriquick-desh, Salmoquick-desh. Numerous classical and chromogenic media for food microbiology.

https://www.microkit.es/eng/fichas/Listeriquick%20&%20Salmoquick%202021%20EN.pdf

https://www.microkit.es/eng/fichas/EXCLUSIVE%20MICROKIT%20PRODUCTS%20FOR%20FOOD%20ANALYSIS,%20WITH%20THEIR%20DIFFERENTIAL%20ADVANTAGES.pdf

https://www.microkit.es/eng/fichas/pca%20cromogenic%20EN.pdf

https://www.microkit.es/eng/fichas/RAPID%20SABOURAUD%20RYM%20AGAR.pdf

https://www.microkit.es/eng/fichas/mugplus%20EN.pdf

https://www.microkit.es/eng/fichas/cromokit%20chromogenic%20media%202021%20EN.pdf

https://www.microkit.es/eng/fichas/MICROKIT%20media%20EN.pdf

https://www.microkit.es/eng/fichas/Cromocytogenes%20EN.pdf

https://www.microkit.es/eng/fichas/SALMONELLA-SHIGELLA%20MICROKIT%20BROTH.pdf

https://www.microkit.es/eng/fichas/CROMOSALM%20EN.pdf

https://www.microkit.es/eng/fichas/BUFFERED%20PEPTONE%20NEUTRALIZING%20WATER%20EN.pdf

 

7-Water Microbiology:

The shelf life of the prepared plates is short. Microbiology of water, beverages and confirmation of pathogens by streaking after enrichment: Hermetic long-life plates.

https://www.microkit.es/eng/fichas/Exclusive%20MICROKIT%20products%20for%20water%20with%20their%20differential%20advantages.pdf

https://www.microkit.es/eng/fichas/BACTERIOFAGOS%20SCHOLTEN%20AGAR.pdf

https://www.microkit.es/eng/fichas/plaquis%20hermetic%20EN.pdf

 

8-Water microbiology in situ:

How do I control the waters in situ? Are we condemned to using only chlorine kits?: Mini-Drinking Water Kit, Vibrio cholerae P/A vials, 100 g P/A bottles

https://www.microkit.es/eng/fichas/drinking%20water%20kit%20EN.pdf

https://www.microkit.es/eng/fichas/swimming%20pool%20kit.pdf

https://www.microkit.es/eng/fichas/P-A%20kits%20in%20sampler%20bottles%20EN.pdf

https://www.microkit.es/eng/fichas/P-A%20powder%20vials%202021.pdf

https://www.microkit.es/eng/fichas/P-A%20Kits%20in%20100%20g%20bottles%20EN.pdf

https://www.microkit.es/eng/fichas/Dry%20Plates%202021%20EN.pdf

https://www.microkit.es/eng/fichas/Exclusive%20MICROKIT%20products%20for%20water%20with%20their%20differential%20advantages.pdf

 

9-Waters-Clostridium:

From the detection of Clostridium perfringens by membrane filtration (with 50% false negatives and a 1-3 log reduction in counts, due to oxygenation during analysis), to the use of Quanti-PA bags that eliminate all these problems, obtaining results with maximum precision, accuracy and linearity. And saving the use of additional atmospheres for anaerobes.

https://www.microkit.es/eng/fichas/QUANTI-PA%20Clostricult%20EN.pdf

 

10-Special dehydrated media for solving multiple problems:

RNA-F bacteriophages (3 and their supplement) and for somatic coliphages (3). BPNW to neutralize the matrix effect in foods. Rapid PCA cromogenic for rapid aerobic counting in only 18-24h. Rapid YM Agar for rapid fungal counting (yeasts and molds) in only 18-36h. Salmonella-Shigella Broth to avoid the false negatives from Rappaport. LPTN Broth to inactivate all current cosmetic preservatives. CUP12Agar to ensure safety against 12 emerging pathogens in cosmetics. Buffered Peptone Neutralizing Water to minimize matrix effect caused by food preservatives and spices…

https://www.microkit.es/eng/fichas/BACTERIOFAGOS%20SCHOLTEN%20AGAR.pdf

https://www.microkit.es/eng/fichas/pca%20cromogenic%20EN.pdf

https://www.microkit.es/eng/fichas/RAPID%20SABOURAUD%20RYM%20AGAR.pdf

https://www.microkit.es/eng/fichas/MICROKIT%20media%20EN.pdf

https://www.microkit.es/eng/fichas/SALMONELLA-SHIGELLA%20MICROKIT%20BROTH.pdf

https://www.microkit.es/eng/fichas/LPT-NEUTRALIZING%20BROTH%20COLOURLESS.pdf

https://www.microkit.es/eng/fichas/CROMOKIT%20CUP-12%20AGAR%20Cosmetic%20Universal%20PathoChrom%20Agar.pdf

https://www.microkit.es/eng/fichas/BUFFERED%20PEPTONE%20NEUTRALIZING%20WATER%20EN.pdf

https://www.microkit.es/eng/fichas/cromokit%20chromogenic%20media%202021%20EN.pdf

https://www.microkit.es/eng/fichas/CROMOSALM%20EN.pdf

https://www.microkit.es/eng/fichas/Cromocytogenes%20EN.pdf

https://www.microkit.es/eng/fichas/mugplus%20EN.pdf

 

We have also developed numerous colony confirmation kits that follow the respective ISO Standards and make you the work easier.

https://www.microkit.es/eng/fichas/m%20ident%20EN.pdf

 

And remember that we also manufacture high precision quantitative microbiological strains

https://www.microkit.es/eng/fichas/QUANTITATIVE%20MICROBIOLOGICAL%20STRAINS%202017.pdf

https://www.microkit.es/eng/fichas/MICROKIT%20export%202024%20Catalogue.pdf

We can design the solution you need to solve the most serious microbiological problem you have: contact us

For more information, contact us at export@microkit.es

If you want to order some MICROKIT product, please send your order to: pedidos@microkit.es